POTENTIAL CHEMOPREVENTIVE AGENT: STUDY OF APOPTOSIS IN THE EXTRACTS OF SPONGE-ASSOCIATED FUNGI FROM YOGYAKARTA AGAINST CERVICAL CANCER HeLa CELL LINE
Abstract
Background: Cervical cancer is one of the leading-cancers affecting women. Cancer drugs that do not originate from natural ingredient, chemotherapy drugs, have side and resistant effects. Thus study about the natural products treating cancer cells is needed. Secondary metabolites isolated from sponge-associated fungi are expected to have a potency to fight cancer cells. In addition, the production of anticancer compounds from microorganisms has several advantages, including rapid growth and can be manipulated to increase productivity. The isolation and testing cytotoxicity against 3 fungal isolates from Yogyakarta have been done on the previous research. All three isolates have a potential candidate as anticancer drug.
Aims: The purpose of this advanced study was studying bioactive compounds induced apoptosis pathway of sponge-associated fungi against cervical cancer HeLa cells.
Methods: This study has been carried out for approximately 5 months. The method conducted in this research including the sponge cultivation (covers growth and isolation of secondary metabolites), the mycelium extraction of fungi, the cytotoxicity assay against HeLa cells using MTT Assay and Apoptosis Staining was to see the induction of apoptosis pathway.
Results: Based on the research showed that ethyl acetate extract from mycelium is 0.22 grams. The cytotoxicity assay from mycelium extract showed IC50 value of 164 μg/mL against HeLa cell line.
Conclusion: The findings is carrying to a possibility to develop the extracts of sponge-associated fungi as candidate of anti-cancer compound. By apoptosis staining, showed the cells coloured green are still alive, and cells undergoing apoptosis have nucleus that appears orange to red. We assuming that the apoptosis was caused by the possibility of peptide compounds that induce apoptosis through the mitochondrial pathway, by increasing the activity of the protein expression of apoptosis, which
are Bcl-2 and Bcl-xl.
References
Bast RC, Kufe DW, and Pollck RE. Holland-Frei cancer medicine. 6th ed. BC Decker; 2000.
Robbory SJ, Anderson MC, and Russel P.Pathology of the female reproduction tract.Philadelpia:Elsevier Science Limited; 2002.
Williams L.A and Anderson A. New symptom measure in chronic myeloid leukemia. european hematology association. 16th
Congress, Haematologica. 2006;96(2): S313-S314.
Devi P, Solimabi W, Cheryl R, and Lisette DS. The sponge-associated bacterium Bacillus licheniformis SAB 1: a source of
antimicrobial compounds.Mar.Drugs. 2010; 8,1203-1212.
Muniarsih T dan Rachmaniar R. Isolasi substansi bioaktif antimikroba dari spons asal Pulau Pari Kepulauan Seribu. Prosidings
Seminar Bioteknologi Kelautan Indonesia I '98. Jakarta: Lembaga Ilmu Pengetahuan Indonesia; 1999.
Muniarsih, T. Metabolit sekunder dari spons sebagai bahan obat-obatan. Oseana; 2003.
Arianto, IA. Sitotoksisitas ekstrak etanolik, metanolik, dan kloroform spons Geodia sp. terhadap sel HeLa (cervical cancer cell line).
Skripsi. Yogyakarta: Fakultas Biologi. Universitas Gadjah Mada; 2012.
Pertiwi, GA, Pramana AAC, Setiawibawa RAA, Zachary AS, dan Putra HE. Saatnya fungi melawan kanker: kajian senyawa bioaktif
fungi yang berasosiasi dengan sponge terhadap proliferasi sel kanker HeLa teruji in vitro. Yogyakarta: Laporan Akhir PKMP. Universitas Gadjah Mada; 2014.
Bewley CA. and Faulkner DJ. Lithistid sponges: star performers or hosts to the stars. Angew Chem (Int Ed Eng).1998 [cited 2015 May 15] ;6(37): 2162–2178.
Rusman, Y. Isolation of new secondary metabolites from sponge-associated and plant-derived endophytic fungi. Disertation. 2006.
Nirwantono, R. Aktivitas antiretrovirus ekstrak etanolik sponge Ancorina sp. terhadap virus HIV-1. Yogyakarta: Skripsi. Fakultas
Biologi. Universitas Gadjah Mada; 2014.
Tarman, K. Biological dan chemical investigations of indonesian marine-derived fungi and their secondary metabolites. Greifswald:
Innagural Dissertation: Ernst-Moritz-Arndt-Universitat; 2011.
Manosroi J, Dhumtanom P. and Manosroi A. Anti-proliferative activity of essential Oil Extracted from Thai Medicinal Plants on KB
an P388 Cell Lines. Elsivier; 2006.
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