POTENTIAL ACCELERATING EFFECT OF Ageratum conyzoides L. LEAVES EXTRACT ON FIBROBLASTS DENSITY OF INCISION WOUND OF MALE WHITE MICE (Mus musculus)
Keywords:
Ageratum conyzoides L., fibroblasts density, incision woundAbstract
Background: Wound treatment using traditional medicine has been known widely in various countries in the world. Ageratum conyzoidesL. is commonly known by the ancient people to treat wound due to its potential anti-inflammatory effect. This study aims determine the effect of Ageratum conyzoidesL. leaves extract on fibroblast density of incision wound of male white mice (Mus musculus).
Methods: This post-test only control group design experimental study used 35 male white mice which were randomly divided into five groups, i.e. negative control group K(-), positive control group K(+) (10% povidone iodine), and treatment group P1, P2, and P3 that were each given billy-goat weed leaf with increasing dose (15%, 30%, and 45% respectively). On each day, the length of the incision was measured by a ruler. After 7 days, the mice were terminated to obtain wound tissue which were used to prepare H&E stained histopathological sections to observe fibroblast density. Non-parametric analyses using Kruskall-Wallis and Mann Whitney test were used to compare the wound length and fibroblasts density.
Results: Lengths of incision wound between all pairs of groups at the 7th day are significantly different (p< 0.05) with group P3 showed the shortest one. Significant differences were also observed in fibroblasts density between group K(-) and K(+), K(-) and P1, K(-) and P2, K(-) and P3, K(+) and P3, P1 and P3, P2 and P3 (p< 0.05) with group P3 showed the highest density among all groups.
Conclusions: Ageratum conyzoidesL. leaves extract 45% has more potential effect than povidone iodine 10% in accelerating healing process by enhancing fibroblasts density.
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References
Rachman UA, Salikunna NA. Pengaruh Lendir Bekicot (Achatina fulica) Terhadap Waktu Penutupan Luka Sayat (Vulnus scissum) Pada Mencit (Mus musculus) (Effect of snail (Achatina fulica) slime on incision wound closure (Vulnus scissum) of mice (Mus musculus)). Kedokteran JI, Program M, Pendidikan S, Tadulako U, Anatomi BP. 2015;2(1).
Oky M. Pengaruh Cairan Cultur Filtrate Fibroblast (CFF) Terhadap Penyembuhan Luka; Penelitian eksperimental pada Rattus Norvegicus Galur Wistar (Effect of Culture Filtrate Fibroblas (CFF) fluid on wound healing; experimental study on Rattus Norvegicus Wistar strain). Kesehat Andalas. 2012:122-127.
Wilson LM. Patofisiologi Konsep Klinis Proses-Proses Penyakit (Patophysiology of clinical concept of diseases). Vol 1. 6th ed. (SA Price LW, ed.). Jakarta: EGC; 2013.
Dewi, Prastiana S. Perbedaan Efek Pemberian Lendir Bekicot (Achatina fulica) Dan Gel BioplacentonTM Terhadap Penyembuhan Luka (Effect comparison of snail (Achatina fulica) slime and BioplacentonTM gel on wound healing). Fakultas Kedokteran Universitas Sebelas Maret. Surakarta.2010.
Sinaga M. Penggunaan Bahan pada Perawatan Luka di RSUD Dr.Djasamen Saragih Pematang Siantar. J Keperawatan Klin. 2012.
Dinkes RI. Kebijakan Obat Tradisional Nasional Tahun 2007. Kesehatan D, Indonesia R. 2007.
Sina I, Sugara TH, Irawadi TT, Suprapto IH, Hanafi M. Uji Aktivitas Antibakteri Fraksi Etil Asetat Daun Tanaman Bandotan (
Ageratum conyzoides L ) (Anti Bacteria Activity of Ethyl Acetate Fraction Bandotan leaf ( Agerantum conyzoides L )). Ilmiah J.
;1(1):88-96.
Afrianti R, Nofiandi D, Ulfa W. Luka Diabetes Melitus Yang Diberikan Sediaan Krim Ekstrak Daun Bandotan (Diabetes Mellitus
wound treated by billy weed extract cream). Scientia. 2016;6(1):50-58
Afrianti R, Yenti R, Monica H. Pengamatan Serabut Kolagen Pada Proses Penyembuhan luka Dalam Sediaan Krim Ekstrak Etanol
Daun Bandotan (Ageratum conyzoides L.) (Observation of collagen tissue of wound healing process of Ageratum conyzoides L. ethanol
extract cream). Scientia.2014
Dalimartha S (dr). Atlas Tumbuhan Obat Indonesia. Jilid 2. jakarta: Trubus Agriwidya; 2006.
Kaur R, Dogra NK. A Review on Traditional Uses , Chemical Constituents and Pharmacology of Ageratum conyzoides L . ( Asteraceae). Int J Phaemaceutical Biol Arch. 2014;5(5):33-45.
Agyare C. Medicinal Plants and Natural Products with Demonstrated Wound Healing Properties. 2016.
doi:http://dx.doi.org/10.5772/46845
Bigliardi PL, Alsagoff SAL, El-Kafrawi HY, Pyon JK, Wa CTC, Villa MA. Povidone iodine in wound healing: A review of current
concepts and practices. Int J Surg. 2017;44:260-268. doi:10.1016/j.ijsu.2017.06.073
Nurdiantini I. Perbedaan Efek Penggunaan Povidine Iodine 10% Dengan Minyak Zaitun Terhadap Penyembuhan Luka Robek
(Laceratum Wound) (Effect difference between povidone iodine 10% use and olive oil on laceration wound healing). 2017;1:223-233.
Anggraeni R. Pengaruh Pemberian Topikal Ekstrak Daun Bandotan (Ageratum conyzoides) Terhadap Peningkatan Jumlah Sel Fibroblas Pada Soket Pasca Pencabutan Gigi Tikus Wistar (Effect of topical use of billy weed (Ageratum conyzoides) leaves extract on fibroblasts
count increase of socket pasca teeth extraction of Wistar Rats). 2013:5.
Vicari K. Wound Healing. Nat Biotechnol. 2013.
Jatana Samreen DLA. Understanding Engineered Nanomaterial Skin Interactions and the Modulatory Effects of Ultraviolet Radiation
Skin Exposure. 2014.
Sumbayak EM. Fibroblas : Struktur dan Peranannya dalam Penyembuhan Luka. (6).
Fawcett DW. Buku Ajar Histologi. 12th ed. (Tambayong ABJ, ed.). Jakarta: EGC; 2002.
M.S Y. Efektivitas Penggunaan Jintan Hitam (Nigella Sativa) dalam Proses Percepatan Penyembuhan Luka Setelah Pencabutan Gigi. Bagian Ilmu Bedah Mulut Fak Kedokt Gigi Univ Hasanudin Makassar. 2014.
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