THE INHIBITION TEST OF FLAVONOID AND TANNIN IN NONI FRUIT (Morinda citrifolia Linn) COMPARED TO CEFTRIAXONE TOWARDS Escherichia coli IN VITRO

Tessa Swesty Islamia, Atik Sutisna, Hikmah Fitriani

Abstract

Background: Escherichia coli is one of the causatives of infection agents. Noni fruit that contains the antibacterial ingredients was predicted as an alternative medicine to reduce the resistance of antibiotics. Noni fruit extract can inhibit the growth of Escherichia coli. The absence of research on the active substances contained in this fruit which plays a role in inhibiting the growth of Escherichia coli encourages the researcher to conduct this research.

Methodology: The research was an experimental laboratory with post-test only control group design. The Escherichia coli grown on Mac conkey agar. Flavonoid and tannin in noni fruit (Morinda citrifolia Linn.) diluted with 3 concentrations, 50%, 70%, 100% and 2 control groups, positive control with ceftriaxone and negative control with Dimethylsulfoxide 10% (DMSO). The treatment group were incubated for 24 hours, then the inhibitory zone formed was measured in mm.

Results: The Kruskall-Wallis test showed significant difference in all concentration. The results obtained from this research showed that the average of inhibition towards Escherichia coli on positive control (22.83 mm) had the largest inhibition zone among flavonoid 100% (18.83mm), flavonoid 70% (14.67 mm), flavonoid 50% (6 mm), tannin 100% (17.33 mm), tannin 70% (10.83 mm), tannin 50% (7.50 mm), and negative control (2 mm).

Conclusion:  Flavonoid and tannin in noni fruit (Morinda citrifolia Linn) were effective for inhibiting the growth of Escherichia coli. The 100% concentration is the most effective concentration that can be promoted as an alternative medicine.

Full Text:

PDF

References

Darmadi. Infeksi Nosokomial: Problematika dan Pengendaliannya. Jakarta: Salemba Medika; 2008.

Nelwan RH, Sudoyo AW, Setiyohadi B, Alwi I, Simadibrata M, Setiati S. Demam: Tipe dan Pendekatan Buku Ajar Ilmu Penyakit Dalam Jilid III. Edisi 5. Jakarta: Interna Publishing; 2009.

Lindgren P K, Karlsson A, Hughes D. Mutation rate and evolution of fluoroquinolone resistance in Escherichia coli isolates from patients with urinary tract infections. Antimicrob Agents Chemother; 2003. 11(2): 3-9.

Katzung BG. Farmakologi Dasar dan Klinik. 12th ed. Jakarta: EGC; 2014.

Thomas ANS. Tanaman Obat Tradisional 2. Cetakan ke-15. Yogyakarta: Kanisius; 2007.

Kementrian Kesehatan Republik Indonesia. Kebijakan Obat Tradisional Nasional. Jakarta: Kemenkes RI; 2007.

Redaksi Trubus. Herbal Indonesia Berkhasiat Bukti Ilmiah dan Cara Racik. Volume 08. Depok: PT. Trubus Swadaya; 2008.

Staf Pengajar Fakultas Kedokteran Universitas Indonesia. Buku Ajar Mikrobiologi Kedokteran. Tanggerang: Binarupa Aksara; 2014.

Purwoko T. Fisiologi Mikroba. Jakarta: Bumi Aksara; 2009.

Forbes BA, Sahm DF, Weissfeld AS. Bailey & Scott’s Diagnostic Microbiology. 12th ed. Philadelphia: Mosby Elsevier; 2007.

Toni H. Khasiat Mengkudu dan Peluang Usahanya. Solo: PT. Pabelan Cerdas Nusantara; 2003.

Ani F, Haniastuti T. Skrining fitokimia dan aktivitas antibakteri ekstrak etanol daun sirsak (Annona muricata Linn) pada Streptococcus mutans ATCC 35668. Majalah Kedokteran Gigi Indonesia. 2017; 3(1): 1-7.

Dahlan S. Statistik untuk kedokteran dan kesehatan. Jakarta: Epidemiologi Indonesia; 2017.

National Committee for Clinical Laboratory Standards. Performance Standards for Antimicrobial Disk Susceptibility Test Sixth Edition: Approved Standards M2-A6. Wayne: NCCLS; 2000.

Ponce AG, Fritz R, del-Valle C, et al. Antimicrobial activity of essential oils on the native microflora of organic Swiss chard. Lebensm Wiss-u Technol 2003;36: 679-684.

Clinical Laboratory Standards Institute. Methods For Dilution Antimicrobial Susceptibility Test For Bacteria That Grow Aerobically: Approved Standard-Ninth Edition. Wayne: PA Clinical and Laboratory Standards Institute; 2015.

Pengajar S. Parasitologi Kedokteran. Vol 8. 4th ed. jakarta: Badan Penerbit FK-UI; 2008.

Cowan M. Plant Products as Antimicrobial Agents, Clinical Microbiology Reviews. Great Britain: Department of Microbiology, Miami University, Oxford; 2005.

Ahmed K. Extended spectrum β-lactamase mediated resistance in Escherichia coli in a tertiary care hospital in Kashmir, India. Journal Microbiology. 2010. 2(2): 5-9.

Todar K. Todar’s Online Textbook Of Bacteriology Staphylococcus aureus and Staphylococcal disease. Departement of Microbiology. 2008. 5(3): 1-4.

Chan-Blanco Y, Vaillant F. The noni fruit (Morinda citrifolia Linn): A review of agricultural research, nutritional and therapeutic properties. Journal of Food Composition and Analysis. 2006; 7(1): 645–654.

Prabuseenivasan S, Jayakumar M, Ignacimuthu S. Research article: In vitro antibacterial activity of some plant essential oils. BMC Complement & Altern Med 2006;6: 1-8

Jantan I, Saputri FC, Qaisar MN, et al. Research article: correlation between chemical composition of Curcuma domestica and Curcuma xanthorrhiza and their antioxidant effect on human low-density lipoprotein oxidation. Evid Based Complementary Altern Med 2012;438356: 1-10.

Junior JNS, Bezzera RP, Porto ARF, Herculano PN, Marques DAV. Effects of Dimethyl sufoxide (DMSO) in Bacterial Strains Growth in MIC Determination. Brazilian Society for Biochemistry and Molecular Biology (SBBq). 2015; 1: 1-2.

Hassan AS. The Antibacterial Activity of Dimethyl Sulfoxide (DMSO) with and without of Some Ligand Complexes of the Transitional Metal Ions of Ethyl Coumarin against Bacteria isolate from burn and wound infection. Journal of Natural Science Research. 2014; 14(19): 21-37.

Pratiwi S. Mikrobiologi farmasi. Jakarta: Erlangga; 2008.

Syariffudin H. Kejadian infeksi saluran kemih di ruang rawat inap anak RSUD dr. Zainoel Abidin Banda Aceh. Sari pediatri. 2012; 14(4). 269-271.

Refbacks

  • There are currently no refbacks.