THE EFFECT OF ORALLY ADMINISTERED CATFISH (Clarias gariepinus) SKIN AND MEAT ON EPITHELIALIZATION THICKNESS AND COLLAGEN DENSITY IN INCISION WOUND OF WISTAR RAT (Rattus norvegicus)

Muhammad Irsyad Baihaqi

Abstract

Background: Alternative medicine using animal is still rare whereas Indonesia have a lot of potential natural resources. Catfish is easily found animal in Indonesia and is common consumed as nutriment sources contains albumin, amino acid and fatty acids which plays an essential role of wound healing process. This study will determine effect of orally administered catfish (Clarias gariepinus) skin and meat on epithelialization thickness and collagen density in incision wound of white male rat (Rattus norvegicus).

Methods: This experimental post-test only group design used 30 white male rat (Rattus norvegicus), that randomly divided into 4 tratment goup and one control group. The treatment group was given 12,5 mg/g W, 25 mg/g W, 37,5 mg/g W and 50 mg/g W dose of catfish (Clarias gariepinus) skin and meat flour, in the other hand the contol group were given aquades. Non-parametric analyses using Kruscall-Wallis and Mann Whitney test were used to compare epithelization thickness and collagen density.

Result: The comparison results of epithelialization thickness between group C with TGI, C with TG2, C with TG3 and C with TG4 showed significant differences (p<0.05). TG4 was the thickest of all groups. Significant differences were also observed in collagen density result between C with TGI, C with TG2, C with TG3 and C with TG4 (p <0,05) TG4 showed the highest density of all groups.

Conclusion: Catfish (Clarias gariepinus) skin and meat flour with 50 mg/200g W dose is effective for wound healing and increasing epithelial thickness and collagen density in Wistar rat (Rattus norvegicus) incision wounds.

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References

Indonesia Health Ministry. Basic Health Research. Jakarta: Indonesia Health Ministry; 2013.

Marvinia S. Effctiveness of Moisture Balance Method on Diabetic Ulcer Patient in Malang Muhammadiyyah University Medical Faculty Health Care. J Saintika Med. 2013:2-15.

Alauddin A. The Effect of Orally Use Snakehead Fish (Channa striata) extract on wistar rat incision wound Fak Kedokt Tanjungpura. 2016.

Nwali BU, Egesimba GI, Ugwu PCO, Ogbanshi ME. Assessment of the nutritional value of wild and farmed Clarias gariepinus. Int J Curr Microbiol Appl Sci. 2015;4(1):179-182.

Zuraida I, Raharjo S, Hastuti P, Indrati R. Catfish ( Clarias gariepinus ): A Potential Alternative Raw Material for Surimi Production. Pakistan Nutr J. 2017;5(1). doi:10.3923/pjn.2017.928.934

Effiong B, Fakunle J. Fatty Acid Composition of Catfish (Clarias Gariepinus) Viscera Oil. Fish Aquat Sci. 2013;8(1):299-301.

Marwadi H. (The Effect of Propolis Extract Locally Aplicated on Collagen Remodeling After Pull out tooth on Guinea Pigs (Cavia cobaya)). 2002.

Sutriyono. Optimization of Binocular Microscope with Micro Digital Camera for Measuring Seminiferous Tubules Epithelium Height. Biol Med Nat Prod Chem. 2016;5(2):41-47. doi:10.14421/biomedich.2016.52.41-47

Acharya G, Mohanty PK. Comparative Haematological and Serum Biochemical Analysis of Catfishes Clarias batrachus and Heteropneustes fossilis with Respect to Sex. J Entomol Zool Stud. 2014;2(6):191-197.

Quain A, Khardori N. Nutrition in Wound Care Management: A Comprehensive Overview. Wounds. 2015;27(12):327-335.

Landen N, Li D, Stahle M. Transition from inflammation to proliferation: a critical step during wound healing. Cell Mol Life Sci. 2016.

Wahyuni IS, Peristiowati Y, Siyoto S. (The Effect of Kutuk Fish (Albumin) on Enhancing Albumin Rate in Hypoalbumin Post Operation Patient in dr. Iskak Hospital Tulunganggung). J Str. 2014;2(3):44-58.

Marcandetti M, Molnar J. Wound Healing and Repair. Emedicine. 2017;14(1):12-20.

Price S. Pathophysiology: Clinical Concepts of Disease Processes. 6th ed. Jakarta: EGC; 2013.

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