THE EFFECTIVENESS OF LEMON JUICE (Citrus limon) ON PURKINJE CELL OF WHITE MALE MICE (Mus musculus) CEREBELLAR CORTEX THAT EXPOSED BY MONOSODIUM GLUTAMATE (MSG)

I Gusti Ayu Novita Afsari, Hikmah Fitriani, Triono Adi Suroso

Abstract

Introduction: A number of studies have indicated that excessive MSG (Monosodium glutamate) consumption can lead to the formation of free radicals that can have a negative effect on purkinje cells in the cerebellar cortex. Lemon fruit is a plant that has benefits as a natural antioxidant because it contains compounds such as flavonoids, vitamin C, citric acid and other substances. This study aims to examine the effect of lemon juice (Citrus limon) to purkinje cells of the cerebellum cortex on male white mice (Mus musculus) which exposed of monosodium glutamate.

Method: This research is an experimental laboratory with a post test only control group design. The research  subjects were 30 white male mice divided into 5 groups: normal control given standard  feed, negative control  given MSG dose 4mg / grBW, group of dose I , dose II , and dose III that were each given lemon juice with doses 3.33ml / kgBW, 6.67ml / kgBW, 13.33ml/ kgBW respectively. The number of purkinje cells is calculated in the cerebellar cortex by HE staining. The results of the study were analyzed by One Way ANOVA followed by the Bonferroni Post hoc test.

Results: The average number of purkinje cells in administration of lemon juice with dosages of 3.33 ml/kgBW, 6.67 ml/kgBW and 13.33 ml /kgBW were 14.10 cells, 16.73 cells and 17.50 cells respectively. Based on the Post Hoc test, the average number of purkinje cells at the dose of 13.33 ml / kgBW was higher than the negative control (p = 0.021)

Conclusion: Lemon juice could be used to reduce negative effect of MSG on purkinje cells of white male mice. Moreover, this finding could be used as reference on further research of benefit of lemon juice.

Full Text:

PDF

References

Badan Penelitian dan Pengembangan Kesehatan. Riset Kesehatan Dasar (RISKESDAS) 2013 (2013 Basic Indonesian Health Research). Lap Nas 2013. 2013:1-384.

Prastiwi D, Djunaidi A, Partadiredja G. High dosage of monosodium glutamate causes deficits of the motor coordination and the number of cerebellar Purkinje cells of rats. Hum Exp Toxicol. 2015;34(11):1171-1179. doi:10.1177/0960327115572706

Ardyanto TD. MSG dan Kesehatan : Sejarah, Efek dan Kontroversinya (MSG and Health: History, Effects and Controversy). Inovasi. 2004;1(16):52-56.

Erdmann NB, Whitney NP ZJ. Potentiation of excitotoxicity in HIV-1 Associated dementia and the Significance of glutaminase. Clin Neurosci Res Elsevier. 2006;6(5):132-142.

Eweka A, Iniabohs FO. Histological Studies Of The Effects Of Monosodium Glutamate On The Cerebellum Of Adult Wistar Rats. Ann Biomed Sci. 2007;5(2):1-5.

Quita SM. Evaluation of lemon fruit extract as an antioxidant agent against histopathological changes induced by cyclophosphamide in the testes of albino mice. Electron physician. 2016;8(1):1824-1831. doi:10.19082/1824

Krisnawan AH, Budiono R, Sari DR, Salim W. Potensi Antioksidan Ekstrak Kulit dan Perasan Daging Buah Lemon (Citrus limon) Lokal dan Impor (Potential Antioxidant of Skin Extract and Juice of Local and Imported Lemon Fruit (Citrus limon) Meat). Pros SEMNASTAN. 2018;0(0):30-34.

Zulfiani, Syafruddin I, Salomo H. Pengaruh Pemberian Vitamin C dan E Terhadap Histologis Ginjal Mencit (Mus musculus L.) yang di Pajankan Monosodium Glutamat (MSG) (Effects of Giving Vitamin C and E on Kidney Histology Mice (Mus musculus L.) Exposed by Monosodium Glutamate (MSG)). Saintia Biol. 2013;1:2-7.

Onaolapo OJ, Onaolapo AY, Akanmu MA, Gbola O. Evidence of alterations in brain structure and antioxidant status following ‘low-dose’ monosodium glutamate ingestion. Pathophysiology. 2016;23(3):147-156. doi:10.1016/j.pathophys.2016.05.001

Anindita R, Soeprobowati TR SN. Potensi Teh Hijau (Camelia sinensis L.) Dalam Perbaikan Fungsi Hepar Pada Mencit Yang Diinduksi Monosodium Glutamat (MSG) (Potential of Green Tea (Camellia sinensis L.) in Repairing Liver Function in Mice Induced by Monosodium Glutamate (MSG)). Bul Anat dan Fisiol. 2011;10(2):15-23.

Razali R. Monosodium Glutamat (MSG) dan Efek Neurotoksisitasnya Pada Sistem Saraf Pusat (Monosodium Glutamate (MSG) and Its Neurotoxicity Effects on the Central Nervous System). Temu Ilmiah Dies Natalis FK Unsyiah ke-33; 2015; Banda Aceh: Fakultas Kedokteran Universitas Syiah Kuala.

Anshori AM, Wiraguna AAGP, Wimpie Pangkahila. Pemberian Oral Ekstrak Kulit Buah Lemon (Citrus limon) Menghambat Peningkatan Ekspresi MMP-1 (Matrix Metaloproteinase-1) dan Penurunan Jumlah Kolagen pada Tikus Putih Galur Wistar Jantan ( Rattus norvegicus ) yang Dipajan Sinar UV-B (Oral administration of Lemon Fruit Extract (Citrus limon) Inhibits Increased Expression of MMP-1 (Matrix Metaloproteinase-1) and Decreased Collagen Amount in Wistar Male (Rattus norvegicus) strain of White Mice Exposed to UV-B Rays). J e-Biomedik. 2017;5(1):3-7.

Zhou T, Zhang Y-J, Xu D-P, et al. Protective Effects of Lemon Juice on Alcohol-Induced Liver Injury in Mice. Biomed Res Int. 2017;2017:1-8. doi:10.1155/2017/7463571

Ataie A, Shadifar M, Ataee R. Review paper: Polyphenolic antioxidants and neuronal regeneration. Basic Clin Neurosci. 2016;7(2):81-90.

Lee JY, Chang MY, Park CH, Kim HY, Kim JH, Son H, Lee YS LS. Ascorbate-induced differentiation of embryonic cortical precursors into neurons and astrocytes. J Neurosci Res. 2003;73(2):156-165. doi:10.1002/jnr.10647

Abdel-Salam OME, Youness ER, Mohammed NA, Morsy SMY, Omara EA, Sleem AA. Citric Acid Effects on Brain and Liver Oxidative Stress in Lipopolysaccharide-Treated Mice. J Med Food. 2014;17(5):588-598. doi:10.1089/jmf.2013.0065

Refbacks

  • There are currently no refbacks.