INFLUENCE OF GIVING BREADFRUIT (Artocarpus altilis (Park) Fosberg.) LEAVES EXTRACT TO DECREASE URIC ACID LEVELS IN WISTARS RATS HYPERURICEMIC

Hagi Wibawa, Hikmah Fitriani, Rama Samara B, Irwan Meidi L

Abstract

Background: Hyperuricemic is a condition where the uric acid levels in blood more than 7 mg/dl. In 2010, Hyperuricemic was included as one of non-communicable disease with incidence rate up to 30% and can cause the mortality. This study aims to examine the effective dose of breadfruit (Artocarpus altilis (Park) Fosberg.) leaves extract to decrease uric acid levels in Wistar rats Hyperuricemic.

Methodology: This study was an experimental with pre-posttest with control group design. 30 Wistar rats aged three months was divided into 2 control groups (K1 and K2) and 3 treatment groups (K3, K4, and K5). Control groups were given purine (K1= standard, K2=high) and aquadest. Differently, treatment groups were given high purine and breadfruit leaf extract (K3=0.25g /KgBW, K4=0.5g /KgBW, K5=0.75g/KgBW). All the treatments were given one time daily in the morning for 7 days. Data were analyzed by the Shapiro-Wilk test followed by the One-Way ANOVA test and then Post-hoc Tamhane test.

Result: The control groups revealed negative average reduction of uric acid in mg/dl (K1= -0.05, K2= -0.09) whereas the treatment groups revealed the positive average reduction of uric acid in mg/dl (K3=2.19, K4= 4.26, K5=5.87). The significant differences (p<0.05) of uric acid levels before and after treatment in each treatment groups had meaning that giving the Breadfruit leaf extract could reduce the uric acid level rats. Post-hoc Tamhane test result showed that the mean decrease of each group was significantly different. Particularly, K5 was the most effective treatment compared with others.

Conclusion: Breadfruit leaves extract with dose of 0.75g/KgBW was the most effective treatment to reduce the uric acid level in blood. Stakeholders in health field should promote the Breadfruit as the traditional medicine method to reduce the uric acid in blood.

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References

Sudoyo, AW: Buku Ajar Ilmu Penyakit Dalam Jilid III (Textbook of Internal MedicineVolume III). 6th Edition. Jakarta: Internal Publishing; 2014. p 3179-3181.

Edwards L. The Role of Hyperuricemia In Vascular Disorders. Curr Opin Rheumatol. 2009;21(2):132-137.

WHO. Global status report on noncommunicable diseases. 2010.

Badan Penelitian dan Pengembangan Kesehatan. Riset Kesehatan Dasar (RISKESDAS) (Health Reasearch and Development Agency) 2013. Lap Nas 2013:94-96

Katzung BG, Masters SB 2014 Farmakologi Dasar & Klinik (Basic Pharmacology & Clinic) Volume 2. Edition 12th . Jakarta: EGC;2014. p 736-737.

Kurniastuty A. Pengaruh Pemberian Fraksi Etil Asetat Ekstrak Etanol 70 % Herba Meniran (Phyllanthus niruri L .) Terhadap Penurunan Kadar Asam Urat Mencit Jantan Galur Balb-C Hiperurisemia (The Effect of Giving Ethyl Acetate Fraction of 70% Ethanol Extract of Menirian Herb (Phyllanthus niruri L .) On Decreasing Uric Acid Levels of Male Mice Balb-C strain Hyperuricemia)[Skripsi]. Surakarta: Universitas Muhammadiyah Surakarta; 2008. p 1-17

S. Kasahara, S. Hemmi. Medicinal Herb Index in Indonesia. PT Eisai Indonesia, Bogor, 1988.

Uthia R, Azlina R, Arifin H. Pengaruh Ekstrak Etanol Daun Sukun ( Artocarpus altilis Parkinson ex F . A . Zorn ) Fosberg ) pada Mencit Putih Jantan Hiperurisemia (Effect of Ethanol Extract of Breadfruit leaves ( Artocarpus altilis Parkinson ex F . A . Zorn ) Fosberg ) on White Mice Hyperuricemia). Prosiding Seminar Nasional & Workshop “Perkembangan Terkini Sains Farmasi & Klinik 5 2015. p 69-76

Hernanti N. Uji Efektivitas Pemberian Ekstrak Daun Sukun (Artocarpus altilis) Terhadap Penurunan Kadar Asam Urat Pada Tikus Putih Jantan Galur Wistar Yang Dibuat Hiperurisemia (Effectiveness Test of Giving Breadfruit Leaf Extract (Artocarpus altilis) Against Decrease in Uric Acid Levels in White Mice Wistar strain made by hyperuricemia) Penerbit FK Unswagati Peneliti Mhs. 2018.

Harborne J. Phytochemistry. Acad Press London; 1993: 89-131.

Lin J. Molecular Modeling of Flavonoids that inhibits xanthine oxidase. Biochemical and Biophysical Research Communications. 2002;294 p 167-172

Cook NC, Samman S. Review Flavonoids-Chemistry, Metabolism, Cardioprotective Effect, And Dietary Sources. J. Nutr. Biochem 7. 1996. p 66-76

Rahmatia TU. Farmaka. Fakultas Farmako Univ Padjajaran. 2016;4:1-13. doi:10.24198/JF.V15I2.13366.

Nagao A, Michiko S, Hidetaka K. 1999.Inhibition of xanthine oxidase by flavonoids. Bioscience,Biotechnology,and Biochemistry J 63:1787–1790

Cos P, et al. Structure-Activity Relationship and Classification of Flavonoids As Inhibitors of Xanthine Oxidase and Superoxide Scavengers. J.Nat. Prod. 1998 61(1):71-76.

Maisuthisakul P. Phenolic Antioxidants from Betel Leaf (Piper betel Linn.) Extract Obtained with Different Solvents and Extraction Time. J Univ Thai Chamb Commer. 2015; 28(2):52-64.

Leng LY, Nadzri NB, Yee KC, Abdul Razak NB, Shaari AR. Antioxidant and Total Phenolic Content of Breadfruit (Artocarpus altilis) Leaves. MATEC Web Conf. 2018;150.

Sadewo VD. Uji Potensi Ekstrak Daun Sukun (Test the Potential of Breadfruif Leaf Extract). Yogyakarta: Universitas Atma Jaya Yogyakarta; 2015.

C.N. Lin, W.L. Shieh. Pyranoflavonoids from Artocarpus communis. Phytochemistry. 31 (8): 2922-2924 (1992).

Syah YM, Achmad SA, Bakhtiar E, Hakim EH, Juliawati LD, Latip J. Dua Flavonoid Tergeranilasi dari Daun Sukun (Artocarpus altilis) (Two Gravitated Flavonoids from Breadfruit Leaves (Artocarpus altilis). J Mat dan Sains. 2006;11(3):100-105.

Sarawek S. Xanthine Oxidase Inhibition and Antioxidant of an Artichoke (Cyanara scolynes L.) and It’s Compound. Disertasi. 2007. Florida: Florida of University.

Azmi U. Efek Ekstrak Etanol Daging Buah Mahkota Dewa (Phaleria macrocarpa [Scheff.] Boerl.) Terhadap Penurunan Kadar Asam Urat Mencit Putih Jantan (Effect of Ethanol Extract of Crown God Fruit (Phaleria macrocarpa [Scheff.] Boerl.) On Decreasing Uwic Acid Levels of Male White Mice). Surakarta: UMS; 2010.

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