• Dela Destiani Aji
  • Ruri Eka Maryam Mulyaningsih
  • Ahmad Fariz MZZ


Background: Excessive consumption of MSG can cause the formation of free radicals in the body. Continuous use of MSG will cause free radical accumulation and oxidative stress in the liver. An antioxidant is one of the substances that has an ability to eliminate free radicals and protect the liver from oxidative stress. Lemon is a fruit that has benefits as a natural antioxidant. This study aims to determine the effect of administering lemon juice on the histopathological appearance of the liver exposed by MSG.

Methodology: This post-test only control group design experimental study used 30 male white mice which were randomly divided into five groups: NC group, C(-) group, D1 group, D2 group, and D3 group. After 7 days of adaptation and randomized grouping, the mice were treated differently based on their group. All groups, except the NC group, were exposed to 4mg/gBW dose of MSG orally for 14 days. On the 15th day, MSG exposure was stopped and after that D1 group, D2 group, and D3 group were given lemon juice with a dose of each group 3.33ml/kgBW, 6.67 ml/kgBW, 13.33 ml/kgBW. The lemon juice was given once a day for 14 days using the gastric sonde. Histopathology examination was analyzed by pathologist in Gunung Jati Hospital, Cirebon, Indonesia

Results: Significant differences of liver histopathology were obtained between the normal control group with the dose 3 group (p = 0.005), the normal control group with the dose 2 group (p = 0.019), the normal control group with the dose group (p = 0.019) and between the negative control groups with dose 3 group (p = 0.027). Dose 3 group has higher liver damage compared to a normal control group and negative control group characterized by the form of parenchymatous degeneration.

Conclusion: Lemon juice has a negative effect on the histopathology appearance of male white mice’s (Mus musculus L.) liver exposed by monosodium glutamate (MSG). Further studies are needed to validate the effect of the active compound and toxicity of lemon juice and to reveal the precise mechanisms on how lemon juice affects the hepatocytes.


Bhattacharya T, Bhakta A, Gosh SK. Long Term Effect of Monosodium glutamate in Liver of Albino Mice after Neo-natal exposure. Nepal Med Coll J: 2011; 13(1): 11-16.

Badan Penelitian dan Pengembangan Kesehatan. Riset Kesehatan Dasar (RISKESDAS) 2013. Lap Nas 2013. 2013:1-384.

Ka He, Shufa D, Pengcheng X, Sangita S, Huijun W, et al. Consumption of monosodium glutamate in relation to incidence of overweight in Chinese adults: China Health and Nutrition Survey (CHNS). Am J Clin Nutr. 2011 Jun; 93(6): 1328–1336. doi: 10.3945/ajcn.110.008870.

Kanti EAA, Susianti. Pengaruh pemberian vitamin c terhadap gambaran histologis hepar mencit jantan dewasa (Mus musculus L.) yang diinduksi monosodium glutamate. Fakultas Kedokteran Universitas Lampung. 2012.

Onyema OO, Farombi EO, Emerole GO, Ukoha Al, Onyeze GO. Effect of vitamin E on monosodium glutamate induces hepatotoxicity and oxidative stress in rats. Indian J Biochem Biophys. 2006 Feb;43(1):20-4.

Sha L, Hor-Yue T, Ning W, Zhang-jin Z, Lixing L, et Al. The Role of Oxidative Stress and Antioxidants in Liver Diseases. Int J Mol Sci. 2015 Nov; 16(11): 26087–26124. doi: 10.3390/ijms161125942.

Nizhar, U.M. Level Optimum Sari Buah Lemon (Citrus limon) sebagai Bahan Penggumpal pada Pembentukan Curd Keju Cottage. Skripsi. Program Studi Teknologi Hasil Ternak. Jurusan Produksi Ternak. Makasar: Fakultas Peternakan. Universitas Hasanudin; 2012.

Hidayah RR. Pengaruh Madu Terhadap Gambaran Mikroskopis Hepar Pada Tikus Wistar Jantan Yang Diinduksi Monosodium Glutamat. 2015.

Eweka AO, Om’Iniabohs. Histological studies of the effects of monosodium glutamate on the liver of wistar rats. The Internet Journal of Gastroenterology, 6(2), 2008, 1528-8323.

Agverianti, T. Pengaruh Pemberian Ekstrak Etanol Rimpang Lengkuas (Alpinia galanga) Terhadap Gambaran Histopatologi Hepar Mencit (Mus musculus L.) Jantan yang Diinduksi Monosodium Glutamate (MSG). (Skripsi). Lampung: Fakultas Kedokteran Universitas Lampung; 2018.

Andreas H, Trianto HF, Ilmiawan MI. Gambaran Histopatologi Regenerasi Hati Pasca Penghentian Pajanan Monosodium Glutamat pada Tikus Wistar. Fakultas Kedokteran Universitas Tanjungpura; 2015; 3(1).

Gilgenkrantz H, de l'Hortet AC. Underdtanding Liver Regeneration From Mechanism to Regenerative Medicine. The American Journal of Pathology, 2018; 188(6): 1316–1327.

Kumar V, Abbas AK, Aster JC. Buku Ajar Patologi Robbins. Edisi 9. Jakarta: Elsevier Saunders; 2015.

Penniston KL, Nakada SY, Holmes RP, Assimos DG; Nakada; Holmes; Assimos ."Quantitative Assessment of Citric Acid in Lemon Juice, Lime Juice, and Commercially-Available Fruit Juice Products". Journal of Endourology. 2008; 22(3):567570. doi:10.1089/end.2007.0304. PMC 2637791. PMID 18290732.

Jamil N, Jabeen R, Khan M, Riaz m, Naeem T, et al. Quantitative Assessment of Juice Content, Citric Acid and Sugar Content in Oranges, Sweet Lime, Lemon and Grapes Available in Fresh Fruit Market of Quetta City. International Journal of Basic & Applied Sciences IJBAS-IJENS. 2015; 15(1): 157301-6464.

Chen X, Lv Q, Liu Y, Deng W. Study on injury effect of food additive citric acid on liver tissue in mice. Cytotechnology. 2014; 66(2): 275–282. doi: 10.1007/s10616-013-9567-1.