THE EFFECTIVENESS OF LEMON JUICE (Citrus limon) TOWARD PYRAMIDAL NEURON CELLS OF MALE WHITE MICE’SCEREBRAL CORTEX THAT EXPOSED BY MONOSODIUM GLUTAMATE (MSG)

Pepi Arifiyani, Hikmah Fitriani, Rachmanda Haryo Wibisono

Abstract

Background: The consumption rate of Monosodium Glutamate (MSG) in Indonesia remains high. Lemon is an antioxidant and neutralizer of free radicals which contains vitamin C and phytochemicals to prevent the long-term effects of MSG to pyramida cells. This study aims to test the effect of oral administration of lemon juice (Citrus limon) to the number of pyramidal neuron cells.  

Methodology: This experimental study employed 30 male white mice aged 2.5 to 3 months. Those mice were randomly divided into five control-groups: Normal Control (KN), Negative Control (K-) was treated with 4 mg/grBB MSG, Dose Group 1 (KD1) was treated with 3.33 ml/kgBB lemon juice, Dose Group 2 (KD2) was treated with 6.67 ml/kgBB lemon juice, and Dose Group 3 (KD3) was treated with 13.33 ml/kgBB lemon juice. Kruskal Wallis test was used to determine the difference in distribution between groups, followed with Post hoc Mann Whitney test.

Results: After 28 days of treatment, the average number of pyramidal neuron cells in dose group 1 (637 cells) was higher than in negative control (589.5 cells) (p=0.078). The average number of pyramidal neuron cells in dose group 3 (482,5 cells) was lower than in the negative control (589.5 cells) (p=0.016). The statistical result shown that there were groups with a significant difference in pyramidal cells number. Mann-Whitney analysis of pyramidal cells number had shown that there was a significant difference between group KD3 and KD1, KD3 and K (-), KD3 and KD2.

Conclusion: Lemon juice (Citrus limon) in precise dose has a beneficial effect to reduce the excitotoxic effect of MSG. Further studies is needed to validate the active compound and toxicity effect of lemon juice.

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